ACE2 : S1 RBD Interaction-Targeted Peptides and Small Molecules as Potential COVID-19 Therapeutics

Figure 2

Optimization of assay and design of peptides. (a) Titration of 6xHis-tagged S1 RBD against a fixed concentration of biotin-tagged ACE2 (∼20 nM) (left). Maximal value was obtained at the hook point before diminishing in signal. Signal-to-background (S/B) ratios at each given concentration used in the titration (right). (b) Interface of the ACE2 and RBD interaction and regions targeted for the rational derivation of peptide fragments (shown in red) that inhibit the binding of the two proteins.

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