Research Article

Antiaging and Antioxidant Bioactivities of Asteraceae Plant Fractions on the Cellular Functions of the Yeast Schizosaccharomyces pombe

Figure 3

Effect of (a) water fraction, (b) chloroform fraction, and (c) water extract on the cell number of stationary phase-yeast cells (11 days of treatment incubation). Yeast cells were grown in YES medium containing an optimum concentration of plant extract/fractions as observed previously in oxidative stress tolerance assay. Yeast cells grown in EMM medium supplemented with DMSO were designated as calorie restriction for the positive control treatment, while yeast cells grown in YES medium supplemented with DMSO only without any extract/fractions addition were used as control. Each culture treatment was then incubated for 11 days and enumerated for cell concentration based on plate count assay using YES agar. Plates were incubated for three days at 30°C.
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