In Vitro Mechanism of Action of Acanthospermum hispidum in Trypanosoma brucei

Figure 2

Effects of A. hispidum on cell morphology and nucleus-kinetoplast distribution of T. b. brucei. (a) Parasites were treated with fractions at the EC50 values and investigated using fluorescence microscopy. (b) Percentage cell population of parasites was calculated from an average count of 200 cells in 10 microscopic fields. AH-HEX = hexane fraction, AH-DCM = dichloromethane fraction, AH-EA = ethyl acetate fraction, AH-AQ = aqueous fraction, NC = negative control, MT = mito-tracker red CMXRos, DIC = differential interference contrast, white arrows (N = nucleus, K = kinetoplast, M = mitochondrion, and F = flagellum of NC); green arrows = mitochondria of fractions, yellow arrows = cell morphology of fractions, and DA = diminazene aceturate (positive control).

(a)

(b)