Passiflora mollissima Seed Extract Induced Antiproliferative and Cytotoxic Effects on CAL 27 Spheroids

Figure 5

Assessment of 3D spheroid viability changes under PME treatment. Representative images of the proapoptotic effect of PME (10, 50, and 100 μg/ml) and 20 μM cisplatin at 72 h. Spheroids stained with Hoechst and propidium iodide (PI). (A1) Merged image (red and blue dyes). Spheroids control does not show double marking. White arrows (⟶) represent apoptotic cells. [2, 3] Fluorescence intensities of Hoechst and propidium iodide in CAL 27 spheroid treated with different concentrations of PME and 20 μM cisplatin at 72 hours using the ImageJ analysis software. Data are shown as median and interquartile range of 3 independent experiments. p < 0.05 vs. control.

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