Stephania japonica Ameliorates Scopolamine-Induced Memory Impairment in Mice through Inhibition of Acetylcholinesterase and Oxidative Stress

Figure 2

Anticholinesterase activity of the crude methanol extract of Sjaponica and its fractions. (a) AChE inhibitory activity. IC₅₀ (µg/mL): DON, 5.21 ± 0.18; CME, 104.13 ± 1.86; CHF, 40.06 ± 0.79; EAF, 159.57 ± 2.57; AQF, 308.57 ± 2.27; PEF, 453.17 ± 4.28. (b) BuChE inhibitory activity. IC₅₀ (µg/mL): GAL, 7.91 ± 0.35; CME, 50.44 ± 1.10; CHF, 18.78 ± 0.69; EAF, 35.30 ± 0.92; AQF, 44.73 ± 1.40; PEF, 100.08 ± 2.12. Results are expressed as mean ± SD (n = 3). Means with different letters (a–f) differ significantly (). CME, crude methanolic extract; CHF, chloroform fraction; EAF, ethyl acetate fraction; AQF, aqueous fraction; PEF, petroleum ether fraction; AChE, acetylcholinesterase; BuChE, butyrylcholinesterase; DON, donepezil; GAL, galantamine.

(a)

(b)