Combination of Chemically-Characterized Essential Oils from Eucalyptus polybractea, Ormenis mixta, and Lavandula burnatii: Optimization of a New Complete Antibacterial Formulation Using Simplex-Centroid Mixture Design

Table 1

Chemical composition of EPEO, OMEO, and LBEO.

No.^a	Compounds^b	Molecular formula	RI^c	RI lit^d	% of relative peak area			Identification
					EPEO	OMEO	LBEO
1	α-Thujene	C10H16	902	905	0.94	—	—	MS, IR
2	Camphene	C10H16	943	943	0.26	—	—	MS, IR
3	α-Pinene	C10H16	948	948	11.20	10.81	0.33	MS, IR
4	3-Octanone	C8H16O	952	948	—	—	0.40	MS, IR
5	β-Myrcene	C10H16	958	958	0.72	—	—	MS, IR
6	-Menthane-1,8-diol	C10H20O2	970	973	—	18.19	—	MS, IR
7	β-Pinene	C10H16	972	972	8.08	3.17	3.73	MS, IR
8	Sabinene	C10H16	975	976	—	2.29	—	MS, IR
9	Yomogi alcohol	C10H18O	998	999	—	1.96	—	MS, IR
10	α-Phellandrene	C10H16	1005	1002	1.76	—	—	MS, IR
11	D-Limonene	C10H16	1018	1020	—	—	1.10	MS, IR
12	Sabinene hydrate	C10H18O	1041	1040	—	—	1.92	MS, IR
13	p-Cymene	C10H14	1042	1041	22.74	—	0.33	MS, IR
14	β-Ocimene	C10H16	1051	1050	—	—	2.51	MS, IR
15	Terpinolene	C10H16	1052	1053	0.87	—	—
16	1,8-cineole	C10H18O	1059	1059	23.75	—	6.57	MS, IR
17	γ-Terpinene	C10H16	1062	1060	7.29	0.97	—	MS, IR
18	Linalool oxide	C10H18O2	1080	1079	—	—	1.21	MS, IR
19	Linalool	C10H18O	1082	1082	0.62	—	24.40	MS, IR
20	Artemisia alcohol	C10H18O	1084	1083	—	1.95	—	MS, IR
21	1-Octenyl-3-acetate	C10H18O2	1109	1115	—	—	0.49	MS, IR
22	Camphor	C10H16O	1121	1122	—	—	8.01	MS, IR
23	(E)-Pinocarveol	C10H16O	1131	1132	0.59	1.17	—	MS, IR
24	Borneol	C10H18O	1138	1134	0.56	—	5.26	MS, IR
25	Fenchyl alcohol	C10H18O	1138	1137	0.48	—	—	MS, IR
26	α-Terpineol	C10H18O	1143	1144	3.11	—	1.71	MS, IR
27	Lavandulol	C10H18O	1146	1148	—	—	1.16	MS, IR
28	Allo-Ocimene	C10H16	1147	1147	0.66	—	0.61	MS, IR
29	Artemisyl acetate	C12H20O2	1153	1152	—	1.75	—	MS, IR
30	Terpinen-4-ol	C10H18O	1177	1177	2.85	1.26	3.79	MS, IR
31	Hexyl butanoate	C10H20O2	1183	1182	—	—	0.84	MS, IR
32	Cryptone	C9H14O	1188	1188	2.69	—	—	MS, IR
33	[E]-3-Caren-2-ol	C10H16O	1188	1188	2.66	—	—	MS, IR
34	Cumin aldehyde	C10H12O	1230	1232	1.57	—	—	MS, IR
35	Carvacrol	C10H14O	1262	1269	0.70	—	—	MS, IR
36	Lavandulyl acetate	C12H20O2	1270	1271	—	—	2.98	MS, IR
37	Linalyl acetate	C12H20O2	1272	1270	—	—	18.68	MS, IR
38	Bornyl acetate	C12H20O2	1277	1273	—	1.39	—	MS, IR
39	Cuminol	C10H14O	1284	1283	0.42	—	—	MS, IR
40	Linalyl propionate	C13H22O2	1319	1318	—	—	0.55	MS, IR
41	Hexyl tiglate	C11H20O2	1331	1331	—	—	0.48	MS, IR
42	α-Terpinyl acetate	C12H20O2	1333	1333	1.35	—	—	MS, IR
43	Geranyl acetate	C12H20O2	1352	1352	—	—	3.75	MS, IR
44	δ-Elemene	C15H24	1365	1462	—	7.18	—	MS, IR
45	β-Elemene	C15H24	1398	1393	—	2.33	—	MS, IR
46	Bornyl isobutyrate	C14H24O2	1412	1402	—	1.03	—	MS, IR
47	α-Bergamotene	C15H24	1430	1430	—	—	0.43	MS, IR
48	γ-Muurolene	C15H24	1435	1435	—	—	0.63	MS, IR
49	β-cis-Farnesene	C15H24	1440	1446	—	—	0.92	MS, IR
50	(Z)-β-Farnesene	C15H24	1440	1443	—	7.58	—	MS, IR
51	α-Farnesene	C15H24	1458	1456	—	2.46	—	MS, IR
52	Alloaromadendrene	C15H24	1461	1461	0.97	—	—	MS, IR
53	Caryophyllene	C15H24	1494	1493	—	3.59	2.21	MS, IR
54	α-Muurolene	C15H24	1499	1500	—	2.97	—	MS, IR
55	β-Bisabolene	C15H24	1500	1500	—	—	0.59	MS, IR
56	Lavandulyl	C15H26O2	1504	1502	—	—	0.84	MS, IR
57	γ-Cadinene	C15H24	1513	1513	—	—	0.28	MS, IR
58	δ-Cadinene	C15H24	1524	1524	—	1.99	—	MS, IR
59	Trans-Nerolidol	C15H26O	1564	1563	—	2.81	—	MS, IR
60	Spathulenol	C15H24O	1575	1572	3.14	1.40	—	MS, IR
61	Caryophylene oxide	C15H24O	1581	1583	—	0.90	0.90	MS, IR
62	Germacrene D	C15H24	1588	1587	—	9.17	—	MS, IR
63	Carotol	C15H26O	1593	1594	—	1.60	—	MS, IR
64	Cedrenol	C15H24O	1604	1604	—	0.91	—	MS, IR
65	Caryophyllene oxide	C15H24O	1632	1630	—	1.63	—	MS, IR
66	τ-Muurolol	C15H26O	1641	1640	—	1.04	—	MS, IR
67	τ-Cadinol	C15H26O	1644	1640	—	—	0.59	MS, IR
68	α-Bisabolol	C15H26O	1682	1683	—	—	1.28	MS, IR
69	cis-Lanceol	C15H24O	1743	1746	—	0.84	—	MS, IR
70	Butanoic acid	C15H22O2	1773	1773	—	1.48	—	MS, IR
Total identified (%)					99.98	95.82	97.47
Monoterpene hydrocarbons					57.18	17.24	8.61
Oxygenated monoterpenes					38.73	27.67	62.49
Sesquiterpene hydrocarbons					0.97	36.27	5.65
Oxygenated sesquiterpenes					3.14	11.13	0.9
Others					—	1.03	22.28
Yield (%, v/w)					2.13	0.7	1.48

^aIn order of elution on HP-5MS, ^bcomponents identified by RI and MS. ^cRetention index determined from alkanes series (C9–C31). ^dRetention index from data libraries (NIST) [30, 31]. Bold values represent the proportions of the major components for each oil.