Advances in Pharmacological and Pharmaceutical Sciences

Research Article

Chemical Profiles and In Vitro Cholinesterase Inhibitory Activities of the Flower Extracts of Cassia spectabilis

Table 2

LC-MS/MS analysis of the ethanolic extract of C. spectabilis flower.


RT^a (mins)	(M + H)⁺	Product ions (m/z)	Formula	Exact mass	Diff (ppm)	Proposed compounds

3.162	138.0548	94.0645, 92.0488, 78.0332, 65.0380, 53.0380	C₇H₇NO₂	138.0550	1.12	6-methylpicolinic acid
3.481	130.0858	103.8087, 84.0803, 67.0534, 56.0491	C₆H₁₁NO₂	130.0863	3.50	Pipecolic acid
7.422	230.1739	212.1630, 194.1522, 95.0849, 70.0645	C₁₂H₂₃NO₃	230.1751	5.08	7-(5,6-dihydroxypiperidin-2-yl)heptan-2-one
8.63	244.1905	226.1789, 208.1679, 185.0062, 95.0846, 70.0647	C₁₃H₂₅NO₃	244.1097	0.90	7-(5-hydroxy-6-(hydroxymethyl)piperidin-2-yl)heptan-2-one
9.232	332.2790	314.2672, 296.2566, 260.2368, 236.2351, 70.0647	C₁₈H₃₇NO₄	332.2795	1.61	Batzellaside A
9.246	346.2572	328.2466, 310.2361, 264.2307, 70.0646	C₁₈H₃₅NO₅	346.2588	4.62	Broussonetine C
11.130	314.2678	296.2570, 278.2464, 236.2360, 135.1156, 95.0850, 70.0646	C₁₈H₃₅NO₃	314.2690	3.72	(−)-7-hydroxycassine
11.584	316.2833	298.2723, 280.2623, 236.2358, 198.1840, 149.1319, 95.0851, 70.0645	C₁₈H₃₇NO₃	316.2846	4.18	Leptophyllin A
12.609	342.2987	324.2889, 306.2780, 95.0849, 81.0693, 70.0648, 55.0538	C₂₀H₃₉NO₃	342.3003	4.59	(−)-7-hydroxyspectaline
13.231	300.2532	282.2411, 264.2309, 182.1526, 123.1158, 95.0849, 70.0648	C₁₇H₃₃NO₃	300.2533	0.40	Leptophyllin B
13.251	358.2942	298.2723, 280.2621, 198.1835, 95.0847, 70.0646	C₂₀H₃₉NO₄	358.2952	2.75	3-O-acetylleptophyllin A
14.044	300.2890	282.2772, 264.2668, 236.2355, 182.1894, 95.0849, 70.0647	C₁₈H₃₇NO₂	300.2897	2.45	(−)-6-iso-carnavaline
14.385	298.2727	280.2627, 252.2303, 198.1837, 149.1310, 123.1158, 95.0848, 70.0645	C₁₈H₃₅NO₂	298.2741	4.55	(−)-cassine
16.015	328.3209	310.3082, 292.2978, 264.2665, 210.2197, 95.0848, 70.0645	C₂₀H₄₁NO₂	328.3210	0.32	(−)-spectalinine
16.419	326.3045	308.2932, 280.2612, 226.2148, 123.1156, 95.0848, 70.0645	C₂₀H₃₉NO₂	326.3054	2.62	(−)-spectaline
17.693	368.3139	326.3025, 308.2928, 226.2136, 95.0848, 70.0645	C₂₂H₄₁NO₃	368.3159	3.04	(−)-3-O-acetylspectaline
18.166	402.2986	280.2620, 252.2302, 212.1992, 184.1668, 149.1310, 70.0645	C₂₅H₃₉NO₃	402.3003	4.15	3(R)-benzoyl-2-(R)-methyl-6(R)-11′-oxododecyl)-piperidine
18.467	300.2893	282.2772, 264.2666, 238.2507, 198.2194	C₁₈H₃₇NO₂	300.2897	1.35	Sphingosine
28.349	582.5430	564.5322, 546.8983, 298.2725, 280.2618, 70.0644	C₃₆H₇₁NO₄	582.5456	4.44	N-(2-hydroxy-docosanoyl)-tetradecasphing-4-enine
28.631	562.5163	453.3398, 340.2575, 264.2669, 69.0691	C₃₆H₆₇NO₃	562.5194	5.46	N-(13Z-docosenoyl)-4E,6E-tetradecasphingadienine
29.616	538.5174	520.5056, 264.2669, 114.0900, 70.0643	C₃₄H₆₇NO₃	538.5194	3.66	N-palmitoylsphingosine
31.276	608.5642	548.5366, 352.3180, 292.2976, 224.2333, 70.0643	C₃₈H₇₃NO₄	608.5612	−4.87	N-(2-hydroxy-tetracosanoyl)-4E,6E-tetradecasphingadienine

Compounds identified in both ethanolic and water extracts.