Advances in Pharmacological and Pharmaceutical Sciences

Research Article

Chemical Profiles and In Vitro Cholinesterase Inhibitory Activities of the Flower Extracts of Cassia spectabilis

Table 3

LC-MS/MS analysis of the water extract of C. spectabilis flower.


RT^a (mins)	(M + H)⁺	Product ions (m/z)	Formula	Exact mass	Diff (ppm)	Proposed compounds

3.499	130.0858	103.8087, 84.0801, 67.0534, 56.0488	C₆H₁₁NO₂	130.0863	3.50	Pipecolic acid
6.113	216.1593	198.1475, 180.1370, 152.1421, 123.0797, 95.0848, 81.0692, 70.0646, 55.0537	C₁₁H₂₁NO₃	216.1594	0.56	5-(5-hydroxy-6-(hydroxymethyl) piperidin-2-yl)pentan-2-one
7.328	230.1747	212.1631, 194.1529, 95.0845, 70.0645, 67.0537	C₁₂H₂₃NO₃	230.1751	1.61	7-(5,6-dihydroxypiperidin-2-yl)heptan-2-one
8.494	244.1905	226.1787, 208.1681, 185.0064, 109.1003, 81.0691, 70.0645	C₁₃H₂₅NO₃	244.1907	0.90	7-(5-Hydroxy-6-(hydroxymethyl) piperidin-2-yl)heptan-2-one
9.107	332.2790	314.2672, 296.2570, 236.2351, 135.1152, 95.0847, 70.0646	C₁₈H₃₇NO₄	332.2795	1.61	Batzellaside A
10.536	314.2687	296.2569, 280.2618, 236.2358, 208.2046, 161.1310, 135.1157, 95.0849, 70.0646	C₁₈H₃₅NO₃	314.2690	0.86	(−)-7-hydroxycassine
11.487	316.2838	298.2723, 280.2620, 236.2358, 198.1836, 149.1313, 121.1002, 95.0849, 70.0646	C₁₈H₃₇NO₃	316.2846	2.59	Leptophyllin A
12.571	340.2839	322.2723, 95.0851, 70.0645	C₂₀H₃₇NO₃	340.2846	2.12	3-O-acetylcassine
12.728	342.2992	324.2878, 306.2774, 121.1004, 95.0849, 70.0646	C₂₀H₃₉NO₃	342.3003	3.13	(−)-7-hydroxyspectaline
13.097	300.2525	282.2407, 264.2302, 236.2350, 182.1524, 147.1153, 123.1155, 95.0848, 70.0645, 55.0536	C₁₇H₃₃NO₃	300.2533	2.73	Leptophyllin B
13.116	358.2942	298.2724, 280.2619, 236.2341, 198.1842, 135.1150, 95.0849, 70.0647	C₂₀H₃₉NO₄	358.2952	2.75	3-O-acetylleptophyllin A
13.718	300.2890	282.2772, 264.2668, 236.2356, 182.1888, 149.1312, 121.1000, 95.0850, 70.0646	C₁₈H₃₇NO₂	300.2897	2.45	(−)-6-iso-carnavaline
14.233	298.2730	280.2627, 252.2306, 198.1837, 149.1311, 123.1158, 95.0848, 70.0645	C₁₈H₃₅NO₂	298.2741	3.54	(−)-cassine
15.165	326.3046	308.2923, 290.2813, 266.2462, 192.1729, 95.0846, 70.0644	C₂₀H₃₉NO₂	326.3054	2.32	6-iso-spectaline
16.172	328.3199	310.3082, 292.2978, 264.2665, 236.2354, 210.2199, 149.1309, 95.0848, 70.0645	C₂₀H₄₁NO₂	328.321	3.37	(−)-spectalinine
16.733	326.3043	308.2932, 280.2612, 226.2148, 177.1620, 149.1308, 123.1156, 95.0848, 70.0644	C₂₀H₃₉NO₂	326.3054	2.32	(−)-spectaline
17.69	368.3148	308.2928, 280.2605, 123.1155, 95.0847, 70.0647	C₂₂H₄₁NO₃	368.3159	3.04	(−)-3-O-acetylspectaline
17.886	318.2992	300.2878, 256.2617, 88.0745, 70.0643, 57.0692	C₁₈H₃₉NO₃	318.3003	3.36	Phytosphingosine
18.058	404.3145	282.2771, 264.2667, 196.2041, 70.0645	C₂₅H₄₁NO₃	404.3159	3.51	6-(10-hydroxy-9-methylundecyl)-2-methylpiperidin-3-yl benzoate
18.424	402.2987	280.2620, 212.1992, 70.0644	C₂₅H₃₉NO₃	402.3003	3.90	3(R)-benzoyl-2-(R)-methyl-6(R)-11′-oxododecyl)-piperidine

Compounds identified in both ethanolic and water extracts.