Development of the RP-HPLC Method for Simultaneous Determination and Quantification of Artemether and Lumefantrine in Fixed-Dose Combination Pharmaceutical Dosage Forms

Table 6

Results of the robustness study.

Condition	Modification	Mean area ± SD	Mean percentage content (%)	% RSD
Flow rate (mL/min)	0.90 (−1 mL/min)	248.25 ± 0.54	98.90	0.22
	1.10 (+1 mL/min)	252.38 ± 0.71	100.53	0.28
Column temperature (°C)	22 (−3°C)	250.05 ± 0.83	99.61	0.33
	30 (+5°C)	247.17 ± 0.09	98.47	0.04
Mobile phase composition (ACN: (0.05%) OPA)	60 : 40	249.36 ± 0.07	99.33	0.03
	65 : 35	248.18 ± 0.14	98.87	0.06

ACN: acetonitrile. OPA: orthophosphoric acid. Acceptance criteria: RSD ≤ 2%.