Development and Characterization of PEGylated Chromatographic Monoliths as a Novel Platform for the Separation of PEGylated RNase a Isomers

Figure 4

Comparison of PEGylated Ribonuclease A separation using different PEGylated monoliths series configurations. Ribonuclease A PEGylation reactions with PEG molecular weights of 5.0, 20.0, and 40.0 kDa were separated using PEGylated monoliths modified with PEGs of 1.0, 10.0, and 20.0 kDa in seven different configurations: 1.0 kDa (black line), 10.0 kDa (red line), 20.0 kDa (light blue line), 10.0-20.0 kDa (yellow line), 20.0-10.0 kDa (green line), 1.0-10.0-20.0 kDa (dark blue line), and 20.0-10.0-1.0 kDa (brown line). Each experiment was carried out by triplicate. Conductivity was also measured and is presented in the chromatograms (gray dashed line).