Towards a Systems Approach in the Genetic Analysis of Archaea: Accelerating Mutant Construction and Phenotypic Analysis in Haloferax volcanii
Figure 3
H. volcanii gene essentiality and growth analysis. Gene essentiality was confirmed by locus-specific PCR (a) HVO_2747, (b) HVO_0253, and (c) HVO_0339. Primers designed to anneal outside of the gene were used as detailed in Supplementary Table 3 and the amplicon size compared to the wild-type parent strain. Amplicon sizes are indicated beside each band. Growth analysis of H. volcanii H26 (wild-type) and the three most severe growth-defected strains in Hv-YPC media (d) and CDM (e). H26, HVO_0916, and HVO_0390 are indicated by diamonds, squares, and circles, respectively. Triangles indicate HVO_2888 (d) or HVO_2478 (e). Each point represents the mean of three independent biological repeats; error bars are plus and minus one standard error.
