RNA-Based Assessment of Diversity and Composition of Active Archaeal Communities in the German Bight

Table 1

Primers used for amplification of the V3–V5 region of the archaeal 16S rRNA [20].

Sample	Primer	Sequence (5′-3′)
		454-Adaptor (Lip-A Kit)	Key	Unique MID	Archaeal 16S rRNA specific
655	ARC344F	CGTATCGCCTCCCTCGCGCCA	TCAG	ACTGTACAGT	ACGGGGYGCAGCAGGCGCGA
658	ARC344F	CGTATCGCCTCCCTCGCGCCA	TCAG	AGACTATACT	ACGGGGYGCAGCAGGCGCGA
659	ARC344F	CGTATCGCCTCCCTCGCGCCA	TCAG	AGCGTCGTCT	ACGGGGYGCAGCAGGCGCGA
660	ARC344F	CGTATCGCCTCCCTCGCGCCA	TCAG	AGTACGCTAT	ACGGGGYGCAGCAGGCGCGA
664	ARC344F	CGTATCGCCTCCCTCGCGCCA	TCAG	ATAGAGTACT	ACGGGGYGCAGCAGGCGCGA
670	ARC344F	CGTATCGCCTCCCTCGCGCCA	TCAG	CACGCTACGT	ACGGGGYGCAGCAGGCGCGA
671	ARC344F	CGTATCGCCTCCCTCGCGCCA	TCAG	CAGTAGACGT	ACGGGGYGCAGCAGGCGCGA
All	ARC915R	CTATGCGCCTTGCCAGCCCGC	TCAG	ACAGTATATA	GTGCTCCCCCGCCAATTCCT