Research Article
Isolation and Molecular Identification of Auxotrophic Mutants to Develop a Genetic Manipulation System for the Haloarchaeon Natrinema sp. J7-2
Table 2
Primers used in this work.
| | Primer name | Sequence (5′-3′) | Restriction site |
| | KpnI-1752 | TAAGGTACCTCGCTGCGATCTTGCAGGGACT | KpnI | | 1752-MfeI | TAACAATTGCGGCGGTCGCGGGCCACTAAA | MfeI | | HindIII-1752 | TTAAAGCTTATGACTCACGAAATCGCCGTCATT | HindIII | | HindIII-4049 | TTAAAGCTTATGAGCGCACTCGAAA | HindIII | | 4049-MfeI | TATCAATTGCCGGTTACGCCACCCCGCG | MfeI | | MfeI-amyH | AATCAATTGATCATATATGCCAACTATTTA | MfeI | | amyH-PstI | AAACTGCAGTAGTGGAAAGCGAGCCAGC | PstI | | leuA-F | ATGGCACGTTATCCGCCATCT | / | | leuA-R | TCAGTCGTCGGCCGGCGT | / | | leuC-F | ATGAGCGCGGAAACGCTG | / | | leuC-R | TCATACCGAGGTCACCTCCT | / | | leuD-F | ATGACGGACGAGGTCGAGATT | / | | leuD-R | TACTCCGCTTCGGGAATCGC | / | | leuB-F | GTGAGTATGACTCACGAAAT | / | | leuB-R | TTACAGTCGGTCGATGAT | / | | dapA-F | GCGACCCAAGAGACCACA | / | | dapA-R | TCATCGCTCATCCTCCG | / | | dapF-F | ATGAATATCCCATTTCAGAAGT | / | | dapF-R | TCACGGCTCGGCCGGGGATT | / | | lysA-F | ATGAACGAAACCCAAACGTT | / | | lysA-R | TATCGGTCGTATTCGTTGTC | / | | dapD-F | ATGAGCGCACTCGAAACCGA | / | | dapD-R | TTACTCCCGCAGCGCGTCCT | / | | dapB-F | ATGACGACGCGAATCGGCGTCAC | / | | dapB-R | TCATTCGCTGATCACGTCTGCGAA | / | | argF-F | ATGACGACCGATTCCGATCC | / | | argF-R | TCACTCGAGCACCCAACTCA | / | | argE-F | ATGAGTGCAACCATGGACGACA | / | | argE-R | TCATTCGTCGTCCTCGCGAA | / | | argD-F | ATGAGCGACCTCGATTTCGT | / | | argD-R | TCATGCGTTGGAGTCGGT | / | | argB-F | GGGCACCCTAACATGACGAC | / | | argB-R | TCATTGGGCCACCTCC | / | | argC-F | ATGGCGGTCGGGACCGAG | / | | argC-R | TTAGGGTGCCCCCACGGG | / | | argXW-F | ATGACCGAATGCGTCGAGTG | / | | argXW-R | TCAGGCGGTCACCTC | / | | argH-F | ATGACCGAGGAGAGCGCTCAC | / | | argH-R | TCAGACATAGCCGTTCACCT | / | | argG-F | GATATGACCCGCGTGGCACT | / | | argG-R | TTACTGGTCGTCTCCCGTCCC | / |
|
|
