Production and Application of a Soluble Hydrogenase from Pyrococcus furiosus

Table 1

Yields of SHI from different expression systems.

Protein	Expression host	Affinity tag	Purification steps	Protein yield (mg)^1	Reference
Native SHI	P. furiosus	—	4	2.5	[6]
Recombinant SHI	E. coli	—	3	0.16	[10]
Strep-tag II SHI	P. furiosus	Strep-tag II	1	17	[11]
9x-His Dimeric SHI	P. furiosus	9x-His tag	2	16	[13]
9x-His SHI	P. furiosus	9x-His tag	1	135	[12]

Protein yield from 100 g of cells (wet weight).