Characterization of the ATP-Dependent Lon-Like Protease in Methanobrevibacter smithii

Table 1

Relative cleavage activities of Lon-like-Ms in the presence of different nucleotides or divalent cations.

Nucleotide	Divalent cation	Peptide hydrolysis (%)	Casein hydrolysis (%)
None	None	5	7
ATP	Mg^2+	100	100
ATP	None	6	4
None	Mg^2+	10	8
AMP-PNP	Mg^2+	92	80
ADP	Mg^2+	64	72
GTP	Mg^2+	90	85
CTP	Mg^2+	131	122
UTP	Mg^2+	64	55
ATP	Ni^2+	20	16
ATP	Ca^2+	83	80
ATP	Mn^2+	89	72
ATP	Co^2+	70	65
ATP	Zn^2+	12	9