VOLN27B: A New Head-Tailed Halovirus Isolated from an Underground Salt Crystal and Infecting Halorubrum

Figure 2

Stability of VOLN27B under different temperature, pH, and salt concentration. Halovirus VOLN27B was heated by different temperature for 10 min prior to conducting plaque formation assay. Virus titer decreased along with the increase of temperature. Viruses were inactivated at 90°C (a). When VOLN27B was kept in NaCl solution with different pH for 12 h, the infectivity was largely blocked when the pH below 5. VOLN27B was relatively stable in alkaline condition (b). VOLN27B was unstable in NaCl solution with the concentration below 12% overnight () (c).

(a)

(b)

(c)