VOLN27B: A New Head-Tailed Halovirus Isolated from an Underground Salt Crystal and Infecting Halorubrum

Figure 3

Influence of chloroform extraction, preservation temperature, and storage time on virus titer. Chloroform extraction slightly reduced virus titer but not in order of magnitude (a). With passage of time from 1 month to 5 months at 4°C, the titer of halovirus VOLN27B in cell-free supernatants decreased from ~10¹¹ to ~10⁸ pfu/mL (b). Virion particles preserved in 4°C were much more stable than in minus 20, 40, and 70°C (c).

(a)

(b)

(c)