Research Article
Structural and Kinetic Characterization of Hyperthermophilic NADH-Dependent Persulfide Reductase from Archaeoglobus fulgidus
Table 2
Steady-state kinetics of AfNpsr under aerobic conditions at 70°C.
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aAll reactions performed with di-, per-, and polysulfide substrates contained 100 μM NADH. bNAD(P)H oxidase activity. No di-, per-, or polysulfides were present. cNR: no reaction. dThe reaction was performed in 1 M Tris, pH 8.7, as in (8). |