Analysis of a Methanogen and an Actinobacterium Dominating the Thermophilic Microbial Community of an Electromethanogenic Biocathode

Figure 3

The methanogenesis pathway and gene expression patterns of methanogenesis-related genes of the Methanothermobacter sp. strain EMTCatA1. (a) Enzymes catalyzing respective reactions in the pathway are indicated in boxes. Fwd: tungsten-containing formyl-MFR dehydrogenase; Fmd: molybdenum-containing formyl-MFR dehydrogenase; Ftr: formyl-MFR:H₄MPT formyltransferase; Mch: N₅N₁₀-methenyl-H₄MPT cyclohydrolase; Mtd: F₄₂₀-dependent N₅N₁₀-methylene-H₄MPT dehydrogenase; Hmd: H₂-dependent N₅N₁₀-methylene-H₄MPT dehydrogenase; Mer: F₄₂₀-dependent N₅N₁₀-methylene-H₄MPT reductase; Mtr: N₅-methyl-H₄MPT methyltransferase; MRI: methyl-CoM reductase I; MRII: methyl-CoM reductase II; Hdr: heterodisulfide reductase; Mvh: methyl viologen-reducing hydrogenase; Frh: F₄₂₀-reducing hydrogenase; MFR: methanofuran; Fd: ferredoxin; H4MPT: tetrahydromethanopterin; CoM-SH: coenzyme M; CoB-SH: coenzyme B. Isofunctional enzymes expressed at higher levels are highlighted in green. (b) TPM-normalized read counts of methanogenesis-related genes in CC condition.

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