Characterization, Expression, and Ligand Binding of LGP2 and MDA5 in Largemouth Black Bass Micropterus salmoides (Lacepède, 1802)

Figure 2

Expression analysis of msLGP2 and msMDA5 genes. (a) Expressions of msLGP2 and msMDA5 genes in normal tissues of largemouth bass. Different tissues of fish (n = 3) were collected to detect gene expression by qPCR, and the levels of gene expression were normalized against that of β-actin. (b) Expressions of msLGP2 in the spleen after polyI:C challenge. (c) Expressions of msMDA5 in the spleen after polyI:C challenge. The fish for polyI:C challenge were i.p. injected with polyI:C (100 μg per 100 g fish) and fish for control were i.p. injected with the same amount of sterilized PBS. At 0 h, 6 h, 12 h, 24 h, 48 h, and 72 h postinjection (hpi), the spleen were sampled and the gene expressions were detected by qPCR. Data were expressed as mean ± SE, with and indicating  < 0.05 and  < 0.01, respectively.

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