Research Article

Dietary Methanolic Extract of Fenugreek Enhanced the Growth, Haematobiochemical, Immune Responses, and Resistance against Aeromonas hydrophila in Nile Tilapia, Oreochromis niloticus

Table 1

Feed ingredients and proximate analysis of different diets.

Feed ingredients (%)Methanolic extract of FS (%)
00.050.10.150.2

Soybean meal (47% Cp)3737373737
Fishmeal (65% Cp)44444
Yellow corn (7.5% Cp)19.419.419.419.419.4
Fish oil0.70.70.70.70.7
Soy oil0.70.70.70.70.7
Rice bran11.211.211.211.211.2
Wheat bran16.516.4516.416.3516.3
Corn gluten66666
Di calcium phosphate0.60.60.60.60.6
Poultry-by meal (60% CP)3.53.53.53.53.5
Vitamins and minerals mixture0.30.30.30.30.3
Choline0.050.050.050.050.05
STAY c-350.050.050.050.050.05
Fenugreek seed extract00.050.10.150.2

Proximate composition (%) (on DM basis)
Dry matter90.1590.2290.2890.2390.31
Crude protein30.130.0830.1130.1330.14
Ether extract6.386.316.346.376.39
Fiber5.125.145.125.135.12
Ash6.156.136.126.146.15
Carbohydrate42.442.5642.5942.4642.51
Gross energy (KJ/g)18.6118.5918.6118.6118.62

Vitamin and mineral mixture (mg/kg of the premix): vitamin B1 (150 mg), vitamin B2 (700 mg), vitamin B6 (500 mg), vitamin B12 (65 mg), biotin (8000 mg), vitamin A (3000 IU), vitamin D3 (550 IU), vitamin E (2950 mg), inositol (300 mg), para-aminobenzoic acid (7500 mg), niacin (30 mg), pantothenic acid (2500 mg), manganese (400 mg), copper (60 mg), iron (300 mg), cobalt (8 mg), iodine (8 mg). STAY c-35: extrusion stable vitamin c for DSM Company (the phosphorous salt of ascorbic acid). Gross energy was calculated based on the values for protein, lipid, and carbohydrate as 23.6, 39.5, and 17.2 KJ/g, respectively, according to [28].