Dietary Methanolic Extract of Fenugreek Enhanced the Growth, Haematobiochemical, Immune Responses, and Resistance against Aeromonas hydrophila in Nile Tilapia, Oreochromis niloticus

Table 1

Feed ingredients and proximate analysis of different diets.

Feed ingredients (%)	Methanolic extract of FS (%)
	0	0.05	0.1	0.15	0.2
Soybean meal (47% Cp)	37	37	37	37	37
Fishmeal (65% Cp)	4	4	4	4	4
Yellow corn (7.5% Cp)	19.4	19.4	19.4	19.4	19.4
Fish oil	0.7	0.7	0.7	0.7	0.7
Soy oil	0.7	0.7	0.7	0.7	0.7
Rice bran	11.2	11.2	11.2	11.2	11.2
Wheat bran	16.5	16.45	16.4	16.35	16.3
Corn gluten	6	6	6	6	6
Di calcium phosphate	0.6	0.6	0.6	0.6	0.6
Poultry-by meal (60% CP)	3.5	3.5	3.5	3.5	3.5
Vitamins and minerals mixture	0.3	0.3	0.3	0.3	0.3
Choline	0.05	0.05	0.05	0.05	0.05
STAY c-35	0.05	0.05	0.05	0.05	0.05
Fenugreek seed extract	0	0.05	0.1	0.15	0.2
Proximate composition (%) (on DM basis)
Dry matter	90.15	90.22	90.28	90.23	90.31
Crude protein	30.1	30.08	30.11	30.13	30.14
Ether extract	6.38	6.31	6.34	6.37	6.39
Fiber	5.12	5.14	5.12	5.13	5.12
Ash	6.15	6.13	6.12	6.14	6.15
Carbohydrate	42.4	42.56	42.59	42.46	42.51
Gross energy (KJ/g)	18.61	18.59	18.61	18.61	18.62

Vitamin and mineral mixture (mg/kg of the premix): vitamin B₁ (150 mg), vitamin B₂ (700 mg), vitamin B₆ (500 mg), vitamin B₁₂ (65 mg), biotin (8000 mg), vitamin A (3000 IU), vitamin D3 (550 IU), vitamin E (2950 mg), inositol (300 mg), para-aminobenzoic acid (7500 mg), niacin (30 mg), pantothenic acid (2500 mg), manganese (400 mg), copper (60 mg), iron (300 mg), cobalt (8 mg), iodine (8 mg). STAY c-35: extrusion stable vitamin c for DSM Company (the phosphorous salt of ascorbic acid). Gross energy was calculated based on the values for protein, lipid, and carbohydrate as 23.6, 39.5, and 17.2 KJ/g, respectively, according to [28].