Research Article

Dietary Methanolic Extract of Fenugreek Enhanced the Growth, Haematobiochemical, Immune Responses, and Resistance against Aeromonas hydrophila in Nile Tilapia, Oreochromis niloticus

Table 2

Primer used for qPCR amplification.

GeneSequence (5′–3′)A.S. (bp)A.T. (°C)Amp. eff(%)Accession no.References

Housekeeping genes
β-actin FCAG​CAA​GCA​GGA​GTA​CGA​TGA​G13561.394.4XM_019351010.2[44]
β-actin RTGT​GTG​GTG​TGT​GGT​TGT​TTT​G
18s rRNA FGGA​CAC​GGA​AAG​GAT​TGA​CAG11058.896.1JF698683.1[42]
18s rRNA RGTT​CGT​TAT​CGG​AAT​TAA​CCA​GAC

Immune-related genes
TNF-α FGGA​AGC​AGC​TCC​ACT​CTG​ATG​A1376193.4[46]
TNF-α RCAC​AGC​GTG​TCT​CCT​TCG​TTC​A
IL-1β FCAA​GGA​TGA​CGA​CAA​GCC​AAC​C1495996.7XM_019365844.2[43]
IL-1β RAGC​GGA​CAG​ACA​TGA​GAG​TGC
TGF-β FTAT​CTG​GGA​TGC​CGA​AAA​C1205593.8NM_001311325.1[47]
TGF-β RGCA​GTG​GCT​CTA​GTG​TCT​GT

A.S.: amplicon size, A.T.: annealing temperature, Amp. Eff: amplification efficiency.