Aquaculture Research / 2023 / Article / Tab 2 / Research Article
Dietary Methanolic Extract of Fenugreek Enhanced the Growth, Haematobiochemical, Immune Responses, and Resistance against Aeromonas hydrophila in Nile Tilapia, Oreochromis niloticus Table 2 Primer used for qPCR amplification.
Gene Sequence (5′–3′) A.S. (bp) A.T. (°C) Amp. eff (%) Accession no. References Housekeeping genes β -actin FCAGCAAGCAGGAGTACGATGAG 135 61.3 94.4 XM_019351010.2 [44 ] β -actin RTGTGTGGTGTGTGGTTGTTTTG 18s rRNA FGGACACGGAAAGGATTGACAG 110 58.8 96.1 JF698683.1 [42 ] 18s rRNA RGTTCGTTATCGGAATTAACCAGAC Immune-related genes TNF-α FGGAAGCAGCTCCACTCTGATGA 137 61 93.4 [46 ] TNF-α RCACAGCGTGTCTCCTTCGTTCA IL-1β FCAAGGATGACGACAAGCCAACC 149 59 96.7 XM_019365844.2 [43 ] IL-1β RAGCGGACAGACATGAGAGTGC TGF-β FTATCTGGGATGCCGAAAAC 120 55 93.8 NM_001311325.1 [47 ] TGF-β RGCAGTGGCTCTAGTGTCTGT
A.S.
: amplicon size, A.T.
: annealing temperature, Amp. Eff
: amplification efficiency.