Dietary Methanolic Extract of Fenugreek Enhanced the Growth, Haematobiochemical, Immune Responses, and Resistance against Aeromonas hydrophila in Nile Tilapia, Oreochromis niloticus

Table 2

Primer used for qPCR amplification.

Gene	Sequence (5′–3′)	A.S. (bp)	A.T. (°C)	Amp. eff(%)	Accession no.	References
Housekeeping genes
β-actin F	CAG​CAA​GCA​GGA​GTA​CGA​TGA​G	135	61.3	94.4	XM_019351010.2	[44]
β-actin R	TGT​GTG​GTG​TGT​GGT​TGT​TTT​G
18s rRNA F	GGA​CAC​GGA​AAG​GAT​TGA​CAG	110	58.8	96.1	JF698683.1	[42]
18s rRNA R	GTT​CGT​TAT​CGG​AAT​TAA​CCA​GAC
Immune-related genes
TNF-α F	GGA​AGC​AGC​TCC​ACT​CTG​ATG​A	137	61	93.4		[46]
TNF-α R	CAC​AGC​GTG​TCT​CCT​TCG​TTC​A
IL-1β F	CAA​GGA​TGA​CGA​CAA​GCC​AAC​C	149	59	96.7	XM_019365844.2	[43]
IL-1β R	AGC​GGA​CAG​ACA​TGA​GAG​TGC
TGF-β F	TAT​CTG​GGA​TGC​CGA​AAA​C	120	55	93.8	NM_001311325.1	[47]
TGF-β R	GCA​GTG​GCT​CTA​GTG​TCT​GT

A.S.: amplicon size, A.T.: annealing temperature, Amp. Eff: amplification efficiency.