Dietary Alpha-Monolaurin for Nile Tilapia (Oreochromis niloticus): Stimulatory Effects on Growth, Immunohematological Indices, and Immune-Related Gene Expressions

Table 2

Oligonucleotide name and sequence of qRT-PCR primers used in the experiment.

Gene	Primer sequence 5′–3′	Accession no.
18s	F:5′-GGTTGCAAAGCTGAAACTTAAAGG-3′; R:5′TTCCCGTGTTGAGTCAAATTAAGC-3′	AF497908.1
INF-γ	F:5′-AAGAATCGCAGCTCTGCACCAT-3′; R:5′-GTGTCGTATTGCTGTGGCTTCC-3	XM_005448319.1
IL-1β	F:5′-CAAGGATGACGACAAGCCAACC-3′; R:5′-AGCGGACAGACATGAGAGTGC-3′	XM_003460625.2
HSP70	F:5′-CATCGCCTACGGTCTGGACAA-3′; R:5′-TGCCGTCTTCAATGGTCAGGAT-3′	′FJ207463.1

INF-γ = interferon gamma; IL-1β = interleukin 1β; HSP70 = heat shock protein 70; 18s rRNA = internal reference gene (house-keeping gene).