Effect of Stocking Density on Culture Efficiency and Physiological Indicators of Largemouth Bass (Micropterus salmoides) under Recirculating Water Conditions in Land-Based Round Ponds

Table 3

Effect of stocking density on free amino acids in muscle tissue of M. salmoides (mg·100 g⁻¹, wet weight).

Amino acids	D1	D2	D3	D4	D5
Essential amino acid
Isoleucine	1.98 ± 0.21	1.45 ± 0.05	1.91 ± 0.90	1.42 ± 0.42	1.74 ± 0.14
Leucine	10.75 ± 1.19	11.33 ± 0.98	13.45 ± 0.36	11.98 ± 2.49	12.41 ± 1.48
Methionine	2.94 ± 0.17	3.44 ± 0.27	4.27 ± 0.04	4.25 ± 1.63	3.92 ± 0.68
Histidine	41.34 ± 11.49	40.80 ± 2.58	40.64 ± 16.64	40.78 ± 0.62	33.99 ± 9.14
Threonine	11.36 ± 0.98	12.43 ± 0.39	13.49 ± 2.77	12.54 ± 1.82	14.61 ± 3.63
Valine	3.58 ± 0.69	3.60 ± 0.31	4.20 ± 1.07	3.39 ± 1.42	4.58 ± 0.79
Phenylalanine	2.59 ± 0.05	3.13 ± 0.61	4.00 ± 0.63	3.72 ± 1.75	3.78 ± 1.15
Lysine	9.91 ± 1.83	9.82 ± 0.47	11.47 ± 1.54	13.36 ± 6.73	9.84 ± 1.66
Nonessential amino acids
Asparticacid^∗	8.93 ± 6.01	5.05 ± 0.25	8.10 ± 0.33	8.64 ± 0.24	9.29 ± 3.15
Glutamic acid^∗	15.07 ± 0.97	18.61 ± 1.28	22.45 ± 6.34	18.53 ± 8.42	21.38 ± 4.90
Serine	8.51 ± 0.97	9.24 ± 1.88	11.08 ± 2.28	9.20 ± 0.14	8.56 ± 0.39
Glycine	57.77 ± 1.55	64.42 ± 24.09	52.28 ± 23.74	44.19 ± 3.70	37.69 ± 9.16
Alamine	28.61 ± 1.07	36.18 ± 2.18	35.87 ± 3.43	29.78 ± 6.90	35.68 ± 6.06
Proline	5.78 ± 1.57	8.97 ± 0.31	11.12 ± 4.33	10.31 ± 4.70	14.21 ± 5.25
Tyrosine	2.49 ± 0.01	2.77 ± 0.22	2.76 ± 0.03	2.78 ± 0.75	2.34 ± 1.17
Σ FAA	211.63 ± 14.83	231.23 ± 26.86	237.11 ± 50.42	214.88 ± 20.60	214.01 ± 12.16
Σ EAA	84.47 ± 12.81	86.00 ± 2.14	93.44 ± 16.86	91.45 ± 3.43	84.86 ± 0.40
Σ UAA	110.38 ± 4.56	124.25 ± 22.93	118.70 ± 26.98	101.14 ± 11.86	104.04 ± 4.95

Note. FAA, free amino acids; EAA, essential amino acids; UAA, umami amino acids.