Effect of Stocking Density on Culture Efficiency and Physiological Indicators of Largemouth Bass (Micropterus salmoides) under Recirculating Water Conditions in Land-Based Round Ponds

Table 4

M. salmoides muscle fatty acid content at the various stocking densities (%).

Fatty acid	D1	D2	D3	D4	D5
C8:0	—	—	—	—	0.03 ± 0.4
C10:0	0.24 ± 0.06^a	0.16 ± 0.04^ab	0.18 ± 0.06^ab	0.14 ± 0.03^ab	0.07 ± 0.09^b
C12:0	—	—	—	—	0.02 ± 0.02
C14:0	1.01 ± 0.01	1.14 ± 0.36	1.33 ± 0.09	1.14 ± 0.15	1.10 ± 0.003
C15:0	0.05 ± 0.01^b	0.19 ± 0.07^a	0.22 ± 0.02^a	0.16 ± 0.05^a	0.18 ± 0.01^a
C16:0	18.13 ± 0.09	17.14 ± 0.24	18.12 ± 0.16	17.47 ± 1.11	18.17 ± 0.14
C17:0	0.18 ± 0.01	0.17 ± 0.04	0.20 ± 0.06	0.21 ± 0.03	0.17 ± 0.03
C18:0	6.01 ± 0.12	5.84 ± 2.05	5.23 ± 0.71	5.11 ± 0.33	5.28 ± 0.22
C20:0	0.24 ± 0.01	0.23 ± 0.01	0.17 ± 0.04	0.22 ± 0.01	0.23 ± 0.07
C22:0	0.18 ± 0.01^a	0.11 ± 0.01^b	0.09 ± 0.01^b	0.09 ± 0.01^b	0.09 ± 0.01^b
C24:0	3.72 ± 0.28	3.53 ± 0.76	2.87 ± 0.31	3.20 ± 0.55	3.75 ± 0.02
∑SFA	29.75 ± 0.20	28.52 ± 2.69	28.33 ± 1.18	27.72 ± 0.45	29.09 ± 0.33
C16:1	1.73 ± 0.05	1.93 ± 0.88	2.59 ± 0.54	2.11 ± 0.59	2.44 ± 0.19
C17:1	0.05 ± 0.001	0.04 ± 0.05	0.07 ± 0.01	0.06 ± 0.02	0.07 ± 0.01
C18:1n9t	0.10 ± 0.01	0.10 ± 0.01	0.14 ± 0.03	0.10 ± 0.03	0.14 ± 0.00
C18:1n9c	16.19 ± 0.61	17.25 ± 5.22	20.77 ± 2.22	17.90 ± 1.58	20.51 ± 1.27
C20:1	0.49 ± 0.01	0.48 ± 0.12	0.54 ± 0.14	0.44 ± 0.05	0.50 ± 0.02
C22:1n9	0.35 ± 0.002	0.33 ± 0.17	0.26 ± 0.08	0.25 ± 0.05	0.24 ± 0.06
C24:1	0.53 ± 0.27	0.19 ± 0.06	0.17 ± 0.04	0.22 ± 0.08	0.19 ± 0.14
∑MUFA	19.44 ± 0.94	20.32 ± 6.05	24.55 ± 2.98	21.08 ± 2.24	24.10 ± 1.58
C18:2n6c	23.32 ± 0.79	26.16 ± 3.26	26.11 ± 0.29	26.13 ± 0.69	23.81 ± 0.60
C18:3n6	0.24 ± 0.11	0.49 ± 0.11	0.43 ± 0.07	0.34 ± 0.33	0.57 ± 0.01
C18:3	1.69 ± 0.06	2.10 ± 0.59	2.15 ± 0.02	2.07 ± 0.06	1.88 ± 0.07
C20:2	0.81 ± 0.00	0.75 ± 0.14	0.72 ± 0.07	0.79 ± 0.06	0.76 ± 0.08
C20:3n6	0.43 ± 0.03	0.35 ± 0.05	0.44 ± 0.02	0.49 ± 0.07	0.57 ± 0.21
C20:3n3	1.60 ± 0.06	1.34 ± 0.70	1.06 ± 0.15	1.32 ± 0.14	1.27 ± 0.31
C22:6n3	22.71 ± 0.48	19.96 ± 6.19	16.22 ± 1.22	20.05 ± 2.10	17.95 ± 0.38
∑PUFA	50.81 ± 1.14	51.15 ± 3.34	47.13 ± 1.80	51.19 ± 2.69	46.81 ± 1.25
∑HUFA	24.75 ± 0.52	21.65 ± 6.94	17.73 ± 1.34	21.86 ± 2.31	19.79 ± 3.51
n-3/n-6	1.01 ± 0.004	0.81 ± 0.35	0.64 ± 0.04	0.79 ± 0.07	0.77 ± 0.004

Note. ∑SFA indicates total saturated fatty acids, ∑MUFA indicates total monounsaturated fatty acids, ∑PUFA indicates total polyunsaturated fatty acids, and ∑HUFA indicates highly unsaturated fatty acids. epa (C20:5n3), eicosapentaenoic acid; DHA (C22:6n3), docosahexaenoic acid. Labeled as essential fatty acids: C18:2n6c, linoleic acid; C18:3n6, gamma linolenic acid.