Prospect of Hydroxyl Radical Exposure during Seawater Bathing to Treat Amoebic Gill Disease in Atlantic Salmon

Table 1

Experimental conditions for CHSE-214, wild amoebae, and clonal NP.

Cell lines or amoebae	H2O2 conc. (mM)	^.OH conc. (mM)	Treatment duration (min)	Media	Temperature (°C)	Further analysis	Remarks
CHSE-214	1	—	30	Freshwater (pH 6.5)	18	Microscopy	Initial experiment to establish a safe maximum limit of in vitro H2O2 concentration towards CHSE-214 as a parent reagent
		—	60
	50	—	240
	100	—	5
	35	35	90	Freshwater (pH 6.5)	18	Flow cytometry	Comparing in vitro viabilities of salmon cell lines exposed to safe H2O2 and ^.OH concentrations at different durations, media, and temperatures via continuous dosing
			120	Freshwater (pH 6.5)	15
			120	Seawater (pH 8, 35 ppt)	18
			240	Seawater (pH 8, 35 ppt)	15
	—	35	60	Seawater (pH 8, 35 ppt)	15	Flow cytometry	Validating previous experiment in seawater at 15°C but shorter treatment duration to check high in vitro viability of salmon cell lines via continuous dosing
Clonal NP	—	35	60	Seawater (pH 8, 35 ppt)	15	Flow cytometry	Validating in vitro lethal dose for clonal NP in seawater at 15°C via one-off dosing
Wild amoebae adhering to CHSE-214	—	35	60	Seawater (pH 8, 35 ppt)	15	Microscopy	Visual check of in vitro lethal dose to wild amoebae in seawater at 15°C via one-off dosing
Clonal NP adhering to CHSE-214	—	35	60	Seawater (pH 8, 35 ppt)	15	Microscopy	Visual check of in vitro lethal dose to clonal NP in seawater at 15°C via one-off dosing