Pharmacokinetic-Pharmacodynamic Analysis of Cephalexin against Streptococcus parauberis in Olive Flounder (Paralichthys olivaceus: Temminck and Schlegel)

Table 1

Primer sequences and polymerase chain reaction conditions were used to detect Streptococcus parauberis.

Pathogen	Primer name	Sequence	Product size (bp)	Reference
S. parauberis	Spa 2,152 Fw	5′-TTT CGT CTG AGG CAA TGT TG-3′	718 bp	[21]
	Spa 2,870 Re	5′-GCT TCA TAT ATC GCT ATA CT-3′
PCR step	Temperature	Reaction time	Cycles
Predenaturation	95°C	5 min	1
Denaturation	95°C	30 s
Annealing	48°C	30 s	30
Extension	72°C	1 min
Final extension	72°C	5 min	1