Effects of Phosphatidic Acid on Mammalian Target of Rapamycin (mTOR) and Metabolic Pathways in Bivalve Mollusk Sinonovacula constricta

Table 1

Primers for real-time RT–PCR.

Target gene	Forward primer (5′ to 3′)	Reverse primer (5′ to 3′)
mTOR	CAGCGGTCTGGTAATGTGAAGG	TAGCAACTTAGGTGACACATACTGG
AMPK	ACCACAGGCACCTCAGTAAACA	AGGATGCGTGCGTGAAGTTA
PK	TCGTGTAATGGCAATAATCG	GTAGAAGCATCGTTCAAGTC
GK	GTTCGCCCGTTTATGCTTGG	CAAGTCCAGGGCGAGAAAGT
GLUT1	CGTTATCCTCGTCGCTTCCA	CCACCATTGCTTCTGTTGGC
G6P	CCCTCGTCTTGTCTGGCATT	CAGCATCCCTGTACACAGCA
PEPCK	GGGAGGACAAGAAGGGAGT	ATTGTATCCCATGAAAGGTCTC
SREBP	GCTCCTACTCTGTTATCCGATTGTG	TCCTGAGACTGGCGAGATCCT
FAS	CTATGTCGAGGCCCATGGTACT	CCGAGTGACCCATGTTCGAT
ACC	TGGATGGCAATGTTGATGA	GGCACTGATGGTAGAGAAG
SCD	CACCGCATCCCCGAAAAATC	AGGCGCAAATTATGGTTGCC
CS	CAGTTCAGTGCTGCCATA	CAAGTTACGGTAGATGATAGAC
NADP	ATGTTGCTAAGGATGTTACC	TTAGGAGATGGACTGTTCTT
β-Actin	CCATCTACGAAGGTTACGCCC	TCGTAGTGAAGGAGTAGCCTCTTT