Research Article

Construction and Evaluation of Recombinant Lactococcus lactis Expressing the vscO Gene as a Safe Live Oral Vaccine against Vibrio alginolyticus

Figure 3

Hereditary stability analysis of Lc-pMG36e-vscO. M: DL2000 DNA marker; v1–v10: PCR product amplified by primers vsco–R/vsco-F; u1–u10: PCR product amplified by primers pMG36e-R/pMG36e-F. v1-10 and u1-10: 5, 10, 15, 20, 25, 30, 35, 40, 45 and 50 generations of Lc-pMG36e-vscO.