Construction and Evaluation of Recombinant Lactococcus lactis Expressing the vscO Gene as a Safe Live Oral Vaccine against Vibrio alginolyticus

Table 1

Primers used in this study.

Primer	Nucleotide sequence (5’-3’)	Accession no.
vscO-R	GCT​CTA​GAG​ATG​ATA​GAA​CGT​TTA​TTA​GA (Xba I)	JX131326.1
vscO-F	CCC​AAG​CTT​TTA​GAT​AAT​GTC​GAC​AGT​GCG (Hind III)
pMG36e-R	GCC​TCC​TCA​TCC​TCT​TCA​TC	Universal primer
pMG36e-F	AAT​ATC​GTA​GCG​CCG​GGG​TA
IL-1β-R	ACG​CTG​CTG​GAC​CTT​TAT​CG	XM_033647455.1
IL-1β-F	ACA​CGG​CTT​TGT​CGT​CTT​TC
TNF-α-R	CTT​CCG​TCG​CTG​TCC​TCA​TGT​G	FJ491411.1
TNF-α-F	GCC​ACA​GGA​TCT​GGC​GCT​ACT​C
MHC-1α-R	TCC​ATC​GTG​GTT​GGG​GAT​GAT​C	FJ896112.3
MHC-1α-F	GCC​GCC​ACG​CTA​CAG​GTT​TCT​A
IL-16-R	TCT​GTT​CTG​CGG​GTT​TAG​C	KP025949.1
IL-16-F	TTC​AGA​TCC​TCC​GTC​CAA​C
β-actin-R	TCA​GGA​TAC​CCC​TCT​TGC​TCT	AY510710.2
β-actin-F	AAATCGCCGCACTGGTTG