Toxic Effects of Copper Sulfate and Trichlorfon on the Intestines of Zebrafish (Danio rerio)

Table 1

The primers for qRT-PCR.

Gene	Sequences	Tm	Amplification efficiency (%)	GenBank accession no
actb	F: AGA​TGG​GAA​CCG​CTG​CCT​CTT	63.88	93.4	NM_131031.2
	R: GTG​GTC​TCG​TGG​ATA​CCG​CAA	62.42
ef1a	F: ACA​GCC​AGA​AGC​GTT​ATG​AGG	60.41	96.2	NM_200009.2
	R: GTC​AGT​TGG​ACG​AGT​TGG​AGG	60.61
ZO1	F: TGC​TCA​GTG​GTA​TCC​CAT​CGT​A	60.69	103.1	XM_021477863.1
	R: GAA​TGG​TGT​CCT​TCA​GTG​CTC​C	61.20
Cldn1	F: AGT​AAA​GTG​GCT​CTG​GCT​GG	59.67	94.1	NM_131770.1
	R: ATA​CAG​CAG​AGC​AGG​GAA​CC	59.46
Oclna	F: CAG​CGA​TTA​CGA​CCG​CAG​AT	60.60	105.7	NM_212832.2
	R: GAG​GCA​CAT​TAG​GAA​CAA​GCA​A	59.18