Effects of Dietary Arginine Level on Growth Performance and Immune Response in Monopterus albus

Table 3

Real-time PCR primer sequences were used to amplify IL-1β and IL-10 gene of M. albushead-kidney.

Gene		Primer sequence (5′--3′)	Annealing temperature	Fragment size
IL-1β	F	AGT​TGT​TTG​CGG​ACC​GAG​AT	58.4	127
	R	GCA​ATC​TGG​CAT​TTC​CTC​ACA	64.2
IL-10	F	GGA​CAA​AGA​TGG​GAA​ACG​AAA	56.4	158
	R	GCC​AGG​TAT​TTG​GTC​TGT​TCA	56.4
18S	F	GTG​GAG​CGA​TTT​GTC​TGG​TTA	57.8	162
	R	CGG​ACA​TCT​AAG​GGC​ATC​AC	57.7

Note. The reference gene 18s is a housekeeping gene of M. albus.