Effects of Dietary Fishmeal Replacement with Soybean Meal on Growth Performance, Digestion, Hepatic Metabolism, Antioxidant Capacity, and Innate Immunity of Juvenile Large Yellow Croaker (Larimichthys crocea)
Table 2
Primer sequences used for real-time quantitative PCR.
Gene
Forward primers
Reverse primers
Accession number
ifn-γ
TCAGACCTCCGCACCATCA
GCAACCATTGTAACGCCACTTA
XM019258900
il-1β
CATAGGGATGGGGACAACGA
AGGGGACGGACACAAGGGTA
XM010736551
il-6
CGACACACCCACTATTTACAAC
TCCCATTTTCTGAACTGCCTCT
XM010734753
il-8
AATCTTCGTCGCCTCCATTGT
GAGGGATGATCTCCACCTTCG
XM010737667.3
il-10
AGTCGGTTACTTTCTGTGGTG
TGTATGACGCAATATGGTCTG
XM010738826
tnf-a
ACACCTCTCAGCCACAGGAT
CCGTGTCCCACTCCATAGTT
NM001303385
m-tor
CACCCACCTTCTTCTTTCAGC
CATTTCCTTGGTTTCCCTCTG
XM027288345.1
rps6
TCAGCGTCCTGAACTTGGTC
CTGAGTGCGCTGTCTCTTGA
XM019267468.2
eif4ebp1
CACGACTACTCCACGACTCC
TGGTCACCCCTGGAATGTTG
XM010732553.3
β-actin
GACCTGACAGACTACCTCATG
AGTTGAAGGTGGTCTCGTGGA
GU584189
ifn-γ, interferon γ; il-1β, interleukin-1β; il-6, interleukin-6; il-8, interleukin-8; il-10, interleukin-10; tnf-a, tumor necrosis factor a; m-tor, mechanistic target of rapamycin; rps6, ribosomal protein S6; eif4ebp1, recombinant eukaryotic translation initiation factor 4e binding protein 1.
