Research Article
Molecular Characterization of Immunoglobulin M (IgM) and Polymeric Immunoglobulin Receptor (pIgR) and Expression Response to Vibrio harveyi Challenge in Leopard Coral Grouper (Plectropomus leopardus)
Figure 7
Recombinant expression and antibacterial activity analysis of recombinant pl-pIgR protein. (a) Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) analysis of recombinant pl-pIgR protein. M protein marker; lane 1: uninduced E. coli total bacteria; lane 2: supernatant of uninduced E. coli; 3: induced E. coli total bacteria; 4: supernatant of induced E. coli; 5: sediment of induced E. coli. The arrow indicates the recombinant pl-pIgR protein. (b) Antibacterial activity of the recombinant pl-pIgR protein in Oxford cup assay.
(a) |
(b) |