Molecular Characterization of Immunoglobulin M (IgM) and Polymeric Immunoglobulin Receptor (pIgR) and Expression Response to Vibrio harveyi Challenge in Leopard Coral Grouper (Plectropomus leopardus)

Table 1

Primers used for amplification, quantitative real-time polymerase chain reaction RT-PCR (qRT-PCR), and protein expression.

Gene	Name	Sequence (5′–3′)	Purpose
pl-IgM	IgM-ORF-F	ATG​TTC​TCT​GTA​GCT​CTG​ATA​CTG	Amplification
	IgM-ORF-R	CTA​TTG​GGG​CTT​GCA​CTT​TTC​AGG
pl-pIgR	pIgR-ORF-F	ATG​CAG​CAG​CTC​TTT​ATA​TTT​G	Amplification
	pIgR-ORF-R	TCA​GTA​CAT​ATG​TAC​TTC​CTC​A
pl-IgM	IgM-RT-F	AGT​GTT​TCC​TCT​GAA​GCC​AT	qRT-PCR
	IgM-RT-R	AAT​GGA​GCC​TGT​CAA​GGC​AA
pl-pIgR	pIgR-RT-F	GAG​GGT​CAG​CCC​CTC​ACA​GT	qRT-PCR
	pIgR-RT-R	TGG​TGC​AGA​ATT​CCT​TCA​TC
Actin	Actin-RT-F	GAGTAGCCACGCTCTGTC	qRT-PCR
	Actin-RT-R	GCTGTGCTGTCCCTGTA
pl-pIgR	pIgR-exF1	GAT​AAC​CAC​CGA​GGG​AGA​GCT​A	Recombinant protein
	pIgR-exR1	ATG​TCA​CAG​ACA​CTG​GGG​CTG​G