The Fin-Improving Effects of Fucosylated Chondroitin Sulfate from Green and Purple Apostichopus japonicus on Caudal Fin Regeneration of Zebrafish Larvae

Figure 4

The effects of FCS on the mRNA levels of genes involved in the regeneration signaling pathway at 4, 24, 48, and 72 hpa. The average transcription values relative to the nonamputation zebrafish larvae at per time points are indicated by the color gradient and log2 transformed (n = 6). Hash signs signify a significant difference from the nonamputation zebrafish larvae based on two-tailedt-tests (^#). Asterisks signify a significant difference from the corresponding only amputation group, determined by one-way analysis of variance (ANOVA) followed by Tukey’s test (). N, A, G, and P represent normal larvae without amputation, the amputation group, G-FCS treatment after amputation group, and P-FCS treatment after amputation group, respectively. The same as below.Wnt10a, wingless-type MMTV integration site family, member 10A; axin2, axis inhibition protein 2; msx1b, muscle segment homeobox 1b; fgf20a, fibroblast growth factor 20a; bmp2a/2b/4, bone morphogenetic protein 2a/2b/4; igf2b, insulin-like growth factor 2b.