Efficient Production of Doubled Haploids as Isogenic Line Founders via Double Heat Shock Treatment in Willow Gudgeon (Gnathopogon caerulescens)

Figure 2

Comparison of morphology, ploidy, and genotype of hatched larvae in each experimental group of experiments I and II. (aI, aII), morphological normality. There is a significant difference between different symbols. (bI, bII), ploidy composition. , haploid: , diploid; , tetraploid; , n/2n mosaic; , others. (cI, cII), rate of gynogenetic diploids and their genotypes. The proportion of homo individuals (DHs) in diploid hatched larvae, as determined by microsatellite loci analysis, was corrected by accounting for the results of the Gras-Di analysis. Solid bars indicate homozygotes, and open bars indicate heterozygotes. Exclude individuals with genetic contributions from the male parent and undetermined individuals. : these experimental data were excluded from the comparison of mean values because there were no hatched larvae in one or two of the three attempts.