Efficient Production of Doubled Haploids as Isogenic Line Founders via Double Heat Shock Treatment in Willow Gudgeon (<i>Gnathopogon caerulescens</i>)

<div>Cytological stages of gynogenetically developed eggs just before heat shock treatment (HST). (a) 25 min after activation in experiment I, zygote stage; (b) 41 min after activation (15 min after HST1 at 25 min after activation) in experiment I, prometaphase; (c)‒(d) 26 min after activation in experiment II, prophase; (e) 15 min after HST1 in experiment II, early prometaphase; (f) 17.5 min after HST1 in experiment II, late prometaphase; (g) 15 min after HST1 in experiment II, metaphase; (h) 25 min after HST1 in experiment II, early anaphase; (i) 25 min after HST1 in experiment II, late anaphase; (j) 25 min after HST1 in experiment II, telophase. n, nuclear; c, chromosome; k, karyomere; dcb, dense chromatin body; p, mitotic pole. Scale = 20 <i>μ</i>m.</div>

Aquaculture Research

fig3

Figure 3

Figure 3: Efficient Production of Doubled Haploids as Isogenic Line Founders via Double Heat Shock Treatment in Willow Gudgeon (<i>Gnathopogon caerulescens</i>) 

Figure 3 | Efficient Production of Doubled Haploids as Isogenic Line Founders via Double Heat Shock Treatment in Willow Gudgeon (Gnathopogon caerulescens) 