Efficient Production of Doubled Haploids as Isogenic Line Founders via Double Heat Shock Treatment in Willow Gudgeon (Gnathopogon caerulescens)

Table 1

Microsatellite primers used in gynogenetic experiments for willow gudgeon.

Target		Sequence	Ta (°C)	Allele size range†	No. of alleles detected†
Gelel 01	F:	TCA​GAT​TTA​GAT​AAC​CGG​AAA​C	58	218–257	8
	R:	CCG​AGC​CTT​TCT​CAT​TCT​TA
Gelel 05	F:	CTG​AGG​CTT​TGC​ATA​TGT​AT	58	97–172	11
	R:	ATT​CAG​AGT​CGT​CAC​AGG​TG
Gelel 06	F:	CGC​TGC​TGT​CAC​TTC​TGT​TT	61	81–114	12
	R:	GGC​CAG​AAG​AGC​TAA​TTA​TA
Gelel 07	F:	CTC​AGT​GTC​GGT​CGG​TTG​GA	59	175–248	12
	R:	GAG​CGT​TTG​GTC​TAG​TTC​AG
Gelel 10	F:	AGC​ACG​GAA​ACC​AGT​CAT​AA	60	164–224	9
	R:	GTC​TGT​AAC​CAG​GGG​CAA​C
Gelel 11	F:	ACA​AAT​AAA​TAG​AGC​AAA​TCA​CGG	64	264–310	9
	R:	AAA​AGT​GGG​TGG​GAC​AGG​TG
Gelel 12	F:	CAG​TTT​GTG​CTT​GTT​AGA​CC	60	252–310	15
	R:	CTT​TGA​GAT​GAA​TGT​GGG​AC
Gelel 13	F:	GAT​AAC​CCC​AGC​AGA​TTC​AT	62	125–234	11
	R:	AGG​CTC​ACT​ATT​TTC​GCA​TT

, annealing temperature (Ta) was modified from the original data reported by Koizumi et al. [21]. †, size, and the number of alleles detected in this study.