Efficient Production of Doubled Haploids as Isogenic Line Founders via Double Heat Shock Treatment in Willow Gudgeon (Gnathopogon caerulescens)

Table 3

Survival rate under various heat shock treatments in each experiment.

Exp. no	Type of development	Timing of HST		N	Number of eggs used	Survival rate at 1 daf (%)	Hatched larvae (%)
		1st	2nd
I	IC	—	—	6	158 ± 66	73.2^a	55.4^a
	GC	—	—	6	133 ± 26	61.0 ± 10.4^ab	38.2 ± 6.3^b
	Single HST	23	—	3	124 ± 57	33.5 ± 16.3^cde	15.4 ± 11.7^c
		25	—	6	130 ± 64	33.1 ± 10.6^cde	12.0 ± 3.8^cdef
		26	—	3	165 ± 102	33.9 ± 5.6^cde	12.4 ± 6.3^cdef
		27	—	6	124 ± 27	37.2 ± 14.2^cd	14.4 ± 4.6^c
		28	—	3	108 ± 9	43.2 ± 8.9^c	15.2 ± 4.6^c
		29	—	6	103 ± 29	37.0 ± 11.5^cd	13.1 ± 2.6^cd
		31	—	3	68 ± 18	46.6 ± 11.8^bc	18.4 ± 6.9^c
	Double HST	23	39	3	109 ± 52	33.8 ± 7.4^cde	12.8 ± 7.4^cde
		25	41	6	173 ± 81	26.7 ± 9.6^def	7.5 ± 6.2^defg
		26	42	3	152 ± 34	19.7 ± 3.7^efg	2.3 ± 1.1^g
		27	43	6	209 ± 75	25.1 ± 9.4^defg	5.1 ± 5.2^fg
		28	44	3	135 ± 64	11.4 ± 4.2^g	1.3 ± 1.6^g
		29	45	6	104 ± 45	15.8 ± 13.8^fg	2.9 ± 3.5^g
		31	47	3	62 ± 14	15.9 ± 13.8^fg	5.4 ± 6.8^efg
II	IC	—	—	3	103 ± 29	88.5^a	78.4^a
	GC	—	—	3	73 ± 25	77.4 ± 12.2^ab	46.6 ± 15.8^b
	Single HST	26	—	3	75 ± 16	64.4 ± 5.9^bc	32.1 ± 6.5^bc
	Double HST	26	37	3	104 ± 61	42.8 ± 26.9^cd	17.8 ± 13.3^cd
		26	39.5	3	75 ± 12	25.5 ± 17.2^de	4.2^❡
		26	42	3	82 ± 29	15.7 ± 26.4^e	2.3^❡
		26	44.5	3	76 ± 23	11.6 ± 20.1^e	2.6^❡
		26	47	3	103 ± 31	18.0 ± 27.9^e	26.4^❡
		26	49.5	3	83 ± 26	17.4 ± 20.4^e	6.1 ± 7.9^cd
		26	52	3	90 ± 36	34.6 ± 21.2^de	16.8 ± 13.4^d

, min after fertilisation. N, number of replicates of the attempts. ^❡, the variability was not calculated because there were one or two of the three attempts without hatched larvae. These experimental data were excluded from the comparison of mean values. This means within each column that are not sharing a common superscript are significantly different ().