Effects of Probiotics and Its Extracellular Products on the Growth Performance, Immune Response, and Aeromonas hydrophila Resistance of Grass Carp

Table 2

Sequences of primers used for RT-qPCR in this study.

Primer	Primer sequence (5^′−3^′)	Accession no.	Product size (bp)	Amplification efficiency (%)
TNF-α	F: TGATGGTGTCGAGGAGGAAGGC	HQ696609	112	100
	R: TTGAGCGTGAAGCAGACAGCAG
IL-6	F: CAGCAGAATGGGGGAGTTATC	KC535507	134	97.35
	R: CTCGCAGAGTCTTGACATCCTT
IL-10	F: GCAACAGAACATCAATAGTCCTT	HQ388294	251	91.36
	R: CACCCTTTTCCTTCATCTTTTCA
Nrf2	F: CTGGACGAGGAGACTGGA	KF733814	234	94.69
	R: ATCTGTGGTAGGTGGAAC
TLR8	F: TCACATCGCTTCCAGGTCTC	HQ638214	133	98.74
	R: ACGGTGAAATAATGGGGGTT
TGF-β1	F: CCACTGTAGAACTAAACCAGGAG	EU099588.1	156	99.11
	R: CTGTGATGTTGAACCATATGTGC
β-Actin	F: GGCTGTGCTGTCCCTGTA	XM_051886219	101	99.95
	R: GGGCATAACCCTCGTAGAT