Research Article
Parentage Determination of Chinese Hooksnout Carp (Opsariichthys bidens) Based on Microsatellite DNA Markers
Table 1
Characteristics of the 14 polymorphic microsatellite markers of O. bidens.
| | Primer code | GenBank accession no. | Repeat motif | Primer sequence (5’–3’) | Ta (°C) | Allele size range (bp) | Chromosome |
| | 2Chr25 | OQ689677 | (TG)40 | F: TACCAACCAAAATGCCAACC | 60 | 171–241 | 25 | | R: GCTCCAAGACATTTCTGTGC |
| | 2Chr19 | OQ689678 | (TG)34 | F: ATTCATTCGAAATCGGCACC | 58 | 129–203 | 19 | | R: ACCTTATCCCAGAGACCCAT |
| | 3Chr15 | OQ689685 | (TAT)13 | F: TCGGCCTAACACCATAGATG | 56 | 246–258 | 15 | | R: GGGTCCATTCATCTAACCCT |
| | 3Chr25 | OQ689687 | (TAT)19 | F: GGCTTGACTTGTTGCATGAC | 56 | 242–266 | 25 | | R: GCTCATCAATGGTGCTTACC |
| | 3Chr14 | OQ689684 | (AAT)12 | F: TTAAGGTCGGGTTGTTAGGC | 56 | 223–235 | 14 | | R: GGGTATATCTGAAGGCCCAG |
| | 3Chr3 | OQ689679 | (TAT)12 | F: GGTTACTCGACATTTCGCTG | 56 | 236–245 | 3 | | R: CAAACTCACGTGGCTTCATT |
| | 3Chr7 | OQ689682 | (AAT)15 | F: GTCAAGAAGAGGGAGCACAT | 58 | 231–246 | 7 | | R: GCCTTTGCTATTAGTTATGGGA |
| | 3Chr6-1 | OQ689681 | (TTG)16 | F: ACATCCAGAGGGCAATCATT | 58 | 134–158 | 6 | | R: AGATCAGGCACACTCTCAAA |
| | 3Chr23-1 | OQ689686 | (ATT)15 | F: GAGTCTGACAGTCATCCAGA | 58 | 236–251 | 23 | | R: AACCTGGATCAGTGATGCTC |
| | 3Chr34-1 | OQ689690 | (TAA)11 | F: TGGTGACCATGAAACGTTTG | 58 | 242–251 | 34 | | R: GGCACGTTGCTATGAATGTT |
| | 3Chr26 | OQ689688 | (ATT)15 | F: ATTGGTCTTGGTTCTCAGCT | 56 | 234–249 | 26 | | R: TCGCCTCCTCACTACAATTC |
| | 3Chr4-1 | OQ689680 | (GAT)12 | F: GCTGCTGTCCTCTAGAGTTT | 58 | 233–239 | 4 | | R: CAGTCAGAGAGCGATGTCTT |
| | 3Chr28-1 | OQ689689 | (AAT)12 | F: CTAGCAGAGGACTATGGCAG | 58 | 152–173 | 28 | | R: CTCTTCAGGGTTTGGGCTAT |
| | 3Chr11 | OQ689683 | (TAA)10 | F: CAGCAGACCGAAAAGAAAGC | 56 | 241–253 | 11 | | R: ACACAGGTGGAAACATGTCT |
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Notes: Ta, annealing temperature.
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