Nelfinavir Impairs Glycosylation of Herpes Simplex Virus 1 Envelope Proteins and Blocks Virus Maturation

Figure 5

NFV affects the glycosylation and subcellular localization of viral gene products but does not change the level of expression. (a) Western blots of HSV-1 infected HF cells show increased mobility in gB and gC with NFV treatment, compared to cells that were untreated or treated with indinavir (IDV). Eastern blots show that altered staining by lectins (peanut agglutinin, PNA; ricinus communis agglutinin I, RCA-I; wheat germ agglutinin, WGA; and concanavalin A, ConA) demonstrates reduced overall glycosylation in NFV-treated cells. (b) HSV-1 gB localization (red staining) is altered in NFV-treated HF cells, showing decreased delineation of plasma membrane processes (indicated by white arrows) by IFA compared to untreated cells. Nuclei are stained blue.

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