Phage-Displayed Peptides Selected to Bind Envelope Glycoprotein Show Antiviral Activity against Dengue Virus Serotype 2

Figure 1

Cloning, expression in E. coli, and purification of codon optimized, dengue 2 envelope domain III. (a) SDS-PAGE showing several steps of protein expression and purification. 1, total lysate of cells before induction; 2, total lysate of cell after IPTG induction; 3, protein molecular weight standards; 4, solubilized inclusion bodies before loading into Ni-NTA column; 5, column flow-through; 6-7, two successive washes; 8-9, two successive elution. (b) Activity of purified and refolded recombinant domain III against DENV2 in Vero cells. Protein was refolded using several refolding conditions (RB1 to RB9) before dialysis and filtration for testing against the virus, in parallel with virus alone and ribavirin as a control. Data is presented as the mean and SD of two independent experiments. Condition RB1 was not used in this experiment as protein consistently precipitated.

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