Adaptation and Molecular Characterization of Two Malaysian Very Virulent Infectious Bursal Disease Virus Isolates Adapted in BGM-70 Cell Line

Figure 8

Deduced amino acid sequences of the isolates. Figure 8 shows the deduced amino acid sequences from positions 213 to 293 where both CEE and BGM-70 adapted UPM190 and UPM0081 isolates showed high similarity with the reference vvIBDV from different parts of the world such as UK661, HK46, JNeto-BR, SA-KZN95, Oyo.NIE 96-09, IRAQ12.127-743 but striking differences with the classical, variant, and serotype 2 reference isolates. Note the E249 and E270 (boxed) amino acids present in some of adapted viruses not seen in other vvIBDV except UPM94/273, a Malaysian isolate with unusual pathogenicity and serotype 2 OH strain.