Adaptation and Molecular Characterization of Two Malaysian Very Virulent Infectious Bursal Disease Virus Isolates Adapted in BGM-70 Cell Line

Figure 9

Nucleotide Phylogenetic Tree Analysis. Phylogenetic analysis of the hypervariable region of the VP2 gene of the IBDV segment A genome from nucleotide positions 637 to 876. The preadaptation, CEE, and BGM-70 adapted viruses are located on the red branch. The vvIBDV pathotypes included in the analysis were AJ878898, AF051838, KF241548, AF508177, D49706.1, X92760, AY780423, AF262030.1, AY520910.1, AF092171.1, AY907007, KC352669.1, AY907012.1, AJ586926.1, and DQ916216. JX424079.1, AY462026, EU162087, AY819703, EU544158, AY907014, AY819702, AY819701, and AY963132.1 represent the classical strains, whereas U30818.1 and KP642112.1 represent the serotype 2 sequences. The evolutionary history and distances were inferred using the neighbor-joining method [17] and the Kimura 2-parameter method [18], respectively. Evolutionary analyses were conducted in MEGA7 [19].