Adaptation and Molecular Characterization of Two Malaysian Very Virulent Infectious Bursal Disease Virus Isolates Adapted in BGM-70 Cell Line

Table 1

cDNA synthesis using IBDV RNA templates.

Condition	Temperature (°C)	Time	Cycle
First denaturation	98	2 min	1
Second denaturation	98	30 sec	35
Annealing	56	30 sec	35
Extension	72	1 min	35
Final extension	72	10 min	1