Adaptation and Molecular Characterization of Two Malaysian Very Virulent Infectious Bursal Disease Virus Isolates Adapted in BGM-70 Cell Line

Table 2

PCR cycling conditions used to amplify the synthesized cDNA templates.

Condition	Temperature	Time	Cycle
Initial denaturation	95°C	3 min	1
Denaturation	98°C	20 sec	35
Annealing	60°C	15 sec	35
Extension	72°C	15–60 sec/kb	35
Final extension	72°C	1 min/kb	1