The Study of Viral RNA Diversity in Bird Samples Using De Novo Designed Multiplex Genus-Specific Primer Panels

Figure 1

Individual primer pairs testing. (1, 8, 18, 19, 28, 33, 36) DNA Ladder (100 bp, 200 bp, 300 bp, 400 bp, 500 bp, 800 bp); (2) ZEBOV, primers ebola_f/r; (3) negative control for primers ebola_f/r; (4) MARV, primers marburg_f/r, (5) negative control for primers marburg_f/r; (6) LASV, primers mammarena_f/r, (7) negative control for primers mammarena_f/r; (9) CCHFV, primers nairo_f/r; (10) PRMV, primers nairo_f/r; (11) negative control for primers nairo_f/r; (12) INKV, primers orthobunya_f/r; (13) negative control for primers orthobunya_f/r; (14) DOBV, primers hanta_f/r; (15) negative control for primers hanta_f/r; (16) KEMV, primers orbi_f1/f2/r; (17) negative control for primers orbi_f1/f2/r; (20) MERS CoV, primers alphacorona_f/r; (21) negative control for primers alphacorona_f/r; (22) MERS (Betacoronavirus), primers betacorona_1_f/r; (23) negative control for primers betacorona_1_f/r; (24) MERS (Betacoronavirus), primers betacorona_2_f/r; (25) negative control for primers betacorona_2_f/r; (26) MERS (Betacoronavirus), primers gammacorona_f/r; (27) negative control for primers gammacorona_f/r; (29) TBEV, primers flavi_f/r; (30) YFV, primers flavi_f/r; (31) JEV, primers flavi_f/r; (32) negative control for primers flavi_f/r; (34) RABV, primers lyssa_f/r; (35) negative control for primers lyssa_f/r.