The Study of Viral RNA Diversity in Bird Samples Using De Novo Designed Multiplex Genus-Specific Primer Panels

Table 2

Primers used in the panel along with their structures.

Primer set	Primer name	Sequence 5’-3’
1	ebola_f	GCAATGTTCAAACACTTTGTGARGC
	ebola_r	CTTAACACCATAGCAACGGTTR
2	marburg_f	TGGACGATAGGAAATCGAGCAC
	marburg_r	TGAACTATRTTGCCTGAGTAGTGWG
3	cueva_f	GTGCCAGAACAGTTTGAACTCA
	cueva_r	CCGAATTCTCTGGGTAACACAA
4	mammarena_f	CAATMCTTGAYATGGGWCARGG
	mammarena_r	WGATTTRAACTCTGCAACAAAYCTR
5	lyssa_f	CTKGAYTATGARAARTGGAACA
	lyssa_r	TATGTCGGRCAYARAACCTGRT
6	ledante_f	AAYAATACATGGCCCACWCC
	ledante_r	ARTATTCTCTMARMKCCCARGACAT
7	alphacorona_f	GGYACHACHTCWGGTGATGS
	alphacorona_r	GYTTACGYARRTAACCAWAAWABTC
8	betacorona_f	GTGCWAAGAATAGAGCYCGCAC
	betacorona_r	RTCACAYTTWGGRTAATCCCAACCC
9	gammacorona_f	CCACATCTGCTAATGTTGCR
	gammacorona_r	CAGAAATRTCWGCTACAAGACCYTG
10	orbi_f1	TACCGCARGATMGWATGATGAT
	orbi_f2	TATGTTCCWCARGATCGRATGATG
	orbi_r	TGCGCTCCAWAVCCATTCCA
11	orthoreo_f	GTYTCGGCGCCYCAYACDYT
	orthoreo_r	GCAGTRTGCTCAGTDGARGT
12	seadorna_f	CCRCATGAYGTHATGGCYCC
	seadorna_r	TCACCWGACTTAACWCCWGM
13	henipa_f	GGTCAGARACWYTGGTGGAYGA
	henipa_r	ARTAYGGATCACTRGCCCARTC
14	phlebo_f	GATTYAATCTSTKSARRGCY
	phlebo_r	YTATYWGYTCCAYCCAGTYYTC
15	hanta_f	GCWGATGCAACWAARTGGTC
	hanta_r	YARRTTYCCYTGYARCCART
16	nairo_f	CCTTCTTTTSHGGYATGATGCA
	nairo_r	GAAGTTAACACTGNCGAWGTWGCATG
17	orthobunya_f	CWGAWGARATGATWWSTGARCCWGG
	orthobunya_r	GCACTCCATTTWGACATRTCWG
18	thogoto_f	ATCAARGAYMRRCTGAARAANA
	thogoto_r	TCGATSYGMGGCTTTATDGM
19	cardio_f	MRGGYATGGAYCCMATGGAV
	cardio_r	AAGTTRGARTARTCYACATCRTAGA
20	parecho_f	GGRATYAACCCATAYAARGAYTGGC
	parecho_r	GAYCCTGATGGCATACCRCC
21	flavi_f	CTSCTKTGTGACATMGGDGA
	flavi_r	TACATCTCRTGYGTGGARTTBC
22	alpha_f	ACWCTGTTTGTSAACACWGTVRTYA
	alpha_r	CTYTTYARRGGGTCTGCSACHC